Manipulating megakaryocytes to manufacture platelets ex vivo
نویسندگان
چکیده
منابع مشابه
Manipulating megakaryocytes to manufacture platelets ex vivo
Historically, platelet transfusion has proven a reliable way to treat patients suffering from thrombocytopenia or similar ailments. An undersupply of donors, however, has demanded alternative platelet sources. Scientists have therefore sought to recapitulate the biological events that convert hematopoietic stem cells into platelets in the laboratory. Such platelets have shown good function and ...
متن کاملTowards the Manufacture of Megakaryocytes and Platelets for Clinical Application.
Platelet transfusions are used in standard clinical practice to prevent hemorrhage in patients suffering from thrombocytopenia or platelet dysfunctions. Recently, a constant rise on the demand of platelets for transfusion has been registered. This may be associated with several factors including demographic changes, population aging as well as incidence and prevalence of hematological diseases....
متن کاملConditional overexpression of transgenes in megakaryocytes and platelets in vivo.
Megakaryocyte (MK)-specific transgene expression has proved valuable in studying thrombotic and hemostatic processes. Constitutive expression of genes, however, could result in altered phenotypes due to compensatory mechanisms or lethality. To circumvent these limitations, we used the tetracycline/doxycycline (Tet)-off system to conditionally over-express genes in megakaryocytes and platelets i...
متن کاملDysplastic Platelets and Circulating Megakaryocytes in Chronic
Small circulating megakaryocytes were nuclear. The circulating megakaryocytes identified in four patients with chronic were associated with the presence of myeloproliferative disorders, and their dysplastic platelets. Both findings are morphologic features by light and electron thought to represent involvement of the microscopy are described. The morphomegakaryocytic-platelet line, probably as ...
متن کاملNormal platelets and megakaryocytes are produced in vivo in the absence of thrombopoietin.
Thrombopoietin (TPO) has been established as the major regulator of megakaryocyte and platelet production. In vitro and in vivo studies have demonstrated that TPO affects both megakaryocyte proliferation and maturation. In vitro, TPO has been reported to be essential for full development of megakaryocytes and platelets. These studies are in contrast to results observed in vivo in mice deficient...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Thrombosis and Haemostasis
سال: 2015
ISSN: 1538-7933,1538-7836
DOI: 10.1111/jth.12946